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Introducing mEdit

Transform mutations into CRISPR edits

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Learn About mEdit

HGVS format or genomic coordinates

Input your mutation details using HGVS notation (e.g., NM_000546.5:c.215C>G) or genomic coordinates (e.g., chr17: 7676154C>T).

Upload CSV

Upload your mutation list as a CSV file. Ensure each row follows the correct format: gene name, coordinate, or HGVS notation.
Drag and Drop or click to Upload file
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Configure Advanced Search Parameters

HGVS format or genomic coordinates

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Select Existing Endonuclease Cas Enzyme(s)
Your Custom Endonuclease Editor
Configure Your Own Endonuclease

PAM

Input the protospacer adjacent motif (PAM) sequence required by your custom endonuclease editor (e.g., NGG for spCas9).
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Endonuclease Mutation-to-cut distance  (0-100 bp)
Define the max searchable distance between the mutation and the double strand break site. HDR efficiency rapidly decreases ~10bp.
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Select Existing Base Editors
Your Custom Base Editor
Configure Your Own Base Editor

PAM

Input the protospacer adjacent motif (PAM) sequence required by your custom base editor.
Specify the original base to be replaced by the base editor (e.g., A for an ABE or C for a CBE).
Define the desired base after editing, depending on the editor's function (e.g., G for an ABE).
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Specify the length of the guide RNA spacer (typically 20 bp for Cas9)
Select this option to run an off-target analysis for each output gRNA.
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Set the max number of mismatches allowed between the gRNA and off-target sites.
Define the max RNA bulge size allowed (RNA bulges allow off-target sites to have gaps)
Define the max DNA bulge size allowed (DNA bulges allow off-target sites to have insertions)
Submit with default settings
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Edit Query
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Loading Results
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Gene

GAA

OMIM

505800

NM_000152.5:c.896T>C

ClinVar

188711

dbSNP

dbSNP

ClinGen

CA273939

Endonuclease Editing
Mutation
(HGVS)
NM_000152.5:c.896T
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See full mutation library
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