How mEdit works
mEdit is a CRISPR guide RNA (gRNA) design platform that enables users to formulate editing strategies that can target specific mutations within the human genome
Submit your variants
Users initiate a mEdit analysis by submitting single nucleotide variants (SNVs) or short insertions/deletions (indels) formatted in Human Genome Variation Society (HGVS) 1 notation or as GRCh38 (hg38) genomic coordinates. Guide RNAs can be designed for a default selection of four Cas endonucleases (SpCas9, SaCas9, Cas12a/Cpf1), and Cas12e/CasX) and two base editors (CBE and ABE).
Expand and refine
your search
Users can expand their search by selecting from a broader menu of 23 Cas endonucleases and 29 base editors, or by defining their own custom editor configurations.
The underlying gRNA design algorithm within mEdit incorporates the specific genetic alteration under consideration, ensuring targeted guide selection. Additionally, users may incorporate pangenome reference assemblies 2 to generate gRNAs that are applicable across diverse genetic backgrounds.
Get your
comprehensive results
For each submitted mutation, mEdit outputs a comprehensive list of gRNAs, each annotated with its predicted on-target editing efficiency scores calculated using Azimuth 3 , DeepCas9 4 , and DeepCpf1 5 . For Cas endonucleases gRNAs an out-of-frame (OOF) score 6 is also provided.
Furthermore, for every gRNA mEdit runs an off-target analysis using GuideScan2 7 , which users can choose to run on either hg38 or the full set of pangenome reference assemblies. The off-target editing sites predicted by GuideScan2 are scored for specificity using the cutting frequency determination score (CFD) 8 .
